Computational analyses of Next-Generation Sequencing (NGS) data obtained on Illumina, BGI/MGI or Ion Torrent platforms can be fully customized for your research needs, the scope and the objectives of the study. We apply only up-to-date and well-proven software/pipelines working under Linux environment. The raw sequencing data is accepted in the forms of BCL, FASTQ and BAM files downloadable from a web-cloud repository or delivered by post to our office on a hard drive.
NGS service providers typically deliver unprocessed FASTQ files containing raw sequencing reads. Before the actual data analysis, raw reads have to be trimmed from contaminating adapter sequences, aligned to a proper reference genome or transcriptome, quantified and, finally, converted into VCF tables (DNA-seq) or tables containing raw read counts (RNA-seq). The VCF files and count tables can be further processed using various packages under R/Bioconductor, Python, as well as Excel software (or similar) depending on the researcher needs and the goals.
Both packages include delivery of quality check reports, tables with mapping statistics and the exact scripts used for data processing.
The price for any custom project is calculated based on the hourly rate of a PhD-holding senior scientist in Germany before taxes according to TV-L E13 (Stufe 5).
The standard processing of raw RNA sequencing data (FASTQ-TO-COUNTS) costs 1.10 EUR per million of raw reads plus 11.00 EUR commission per sample. The downstream differential expression analysis will cost additionally 25.00 EUR per sample.
The standard processing of raw DNA sequencing data (FASTQ-TO-VCF) costs 1.20 EUR per million of raw reads plus 15.00 EUR commission per sample. The downstream variants annotation and statistical analysis will cost an additional 30.00 EUR per sample.
* The rates indicated above do not include VAT.
You could first send us a short project description by email or using the contact form below. Upon evaluating your request with we will offer an initial free-of-charge consultation to discuss the details of the project including the deliverables and the price. Alternatively, you could call us directly at +49 171 1908276 to discuss your project details and the terms of the contract.