Recommended sequencing depth

What is the recommended sequencing depth?

WES for genetic disorders

50x

WES for tumor mutations

100x

WGS for genetic disorders

30-50x

WGS for tumor mutations

50x

WTS, mRNA-seq

20 M reads

small RNA-seq

10 M reads

ChIP-seq

20 M reads

Microbiome (16S/18S amplicon sequencing)

30000, 50000 or 100000 tags

BS-seq

30x

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